Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16

Citation
H. Sashiyama et al., Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16, INT J ONCOL, 19(1), 2001, pp. 97-103
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
19
Issue
1
Year of publication
2001
Pages
97 - 103
Database
ISI
SICI code
1019-6439(200107)19:1<97:IOHEKB>2.0.ZU;2-F
Abstract
Transduction of human papillomavirus type 16 (HPV16) E6/E7 into primary cul ture of human esophageal keratinocytes using a recombinant adenovirus prolo nged the life-span, while untreated cells senesced within 14-16 population doublings (PDLs). Up-regulation of telomerase activity and acquisition of s erum-resistant growth were observed in the esophageal keratinocytes with ex tended lifespan between 50 and 100 PDLs, and drastically increased after 10 0 PDLs. A keratinocyte sample with a polymorphism of Pro/Pro at codon 72 of p53 showed resistance to HPV16 E6/E7-induced life-span-extension and immor talization, in contrast to others with p53 polymorphisms of Arg/Arg or Arg/ Pro, which did not. The high efficiency of E6/E7-induction by adenovirus ve ctor also revealed the M1 and M2 stages of keratinocyte immortalization fir st described in this report.