Characterization of two genes, Sig1 and Sig2, encoding distinct plastid sigma factors(1) in the moss Physcomitrella patens: phylogenetic relationships to plastid sigma factors in higher plants

Citation
K. Hara et al., Characterization of two genes, Sig1 and Sig2, encoding distinct plastid sigma factors(1) in the moss Physcomitrella patens: phylogenetic relationships to plastid sigma factors in higher plants, FEBS LETTER, 499(1-2), 2001, pp. 87-91
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
0014-5793 → ACNP
Volume
499
Issue
1-2
Year of publication
2001
Pages
87 - 91
Database
ISI
SICI code
0014-5793(20010615)499:1-2<87:COTGSA>2.0.ZU;2-L
Abstract
We isolated the cDNA for a a factor from the moss Physcomitrella patens, wh ich possesses unusually large N-terminal extension and the conserved subdom ains 1.2-4.2. Phylogenetic analyses indicated that tills nor el a factor an d PpSIGI*(2), a plastid a factor previously identified from Physcomitrella, were classified into SigA and SigB groups, two major classes of higher pla nt plastid a factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel a factor PpSI G1. A transient expression assay using a green fluorescent protein showed t hat the N-terminal region of PpSIG1 acts as a chloroplast-targeting signal, Reverse transcription-PCR experiments showed that light induces the expres sion of the Sig1 and Sig2 genes encoding PpSIC1 and PpSIG2, respectively. T hus, PpSIC1 and PpSIG2 are likely plastid a factors regulating plastid gene expression in response to light signals. (C) 2001 Federation of European B iochemical Societies. Published by Elsevier Science B.V. All rights reserve d.