Acute pancreatitis signals activation of apoptosis-associated and survivalgenes in mice

Citation
G. Gomez et al., Acute pancreatitis signals activation of apoptosis-associated and survivalgenes in mice, EXP BIOL ME, 226(7), 2001, pp. 692-700
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
EXPERIMENTAL BIOLOGY AND MEDICINE
ISSN journal
1535-3702 → ACNP
Volume
226
Issue
7
Year of publication
2001
Pages
692 - 700
Database
ISI
SICI code
1535-3702(200107)226:7<692:APSAOA>2.0.ZU;2-R
Abstract
In experimental models of acute pancreatitis (AP), acinar cell death occurs by both necrosis and programmed cell death or apoptosis. Apoptosis Is an a ctive form of cell death associated with a tightly regulated expression of gene products that are either pro- or antiapoptotic. The sim of this study was to characterize pancreatic mRNA levels by Northern blotting analysis of apoptosis-associated genes used during the course of cerulein-induced AP i n mice. Histone H3 mRNA levels were also examined as an indicator of cell p roliferation. Acinar cell apoptosls was confirmed histologically. The findi ngs show that AP modifies pancreatic mRNA levels of both pro- and antiapopt otic genes simultaneously. Pancreatic bcl(XL), bax, and p53 mRNA levels inc reased significantly in a temporal fashion during induction of AP, Pancreat ic bcl-2 mRNA levels were unchanged during AP, Pancreatic mRNA levels of in sulin-like growth factor-1 (IGF-1), a mitogen and cell survival factor, and Its receptor (IGF-IR) also increased in a temporal fashion during inductio n of AP. In summary, this study indicates that acinar cell death during cer ulein-induced AP in mice can occur by the apoptotic pathway. Since factors promoting and antagonistic for cell survival are activated simultaneously, regulation of acinar cell survival appears complex and dynamic during AP,