Involvement of poly(ADP-ribose) polymerase in trophoblastic cell differentiation during tumorigenesis

Citation
M. Masutani et al., Involvement of poly(ADP-ribose) polymerase in trophoblastic cell differentiation during tumorigenesis, MUT RES-F M, 477(1-2), 2001, pp. 111-117
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
ISSN journal
1386-1964 → ACNP
Volume
477
Issue
1-2
Year of publication
2001
Pages
111 - 117
Database
ISI
SICI code
1386-1964(20010602)477:1-2<111:IOPPIT>2.0.ZU;2-B
Abstract
Poly(ADP-ribose) polymerase (Parp) monitors DNA strand breaks and poly(ADP- ribosyl)ates nuclear proteins using NAD as a substrate. The participation o f Parp in DNA damage responses has been demonstrated by recent studies usin g Parp knockout mice. On the other hand, accumulated evidence has shown tha t Parp is involved in the regulation of gene expression and cell differenti ation. In this study, the role of Parp in tumorigenesis and differentiation was studied with Parp(-/-) embryonic stem (ES) cells. When Parp(+/+), Parp (+/-), and Parp(-/-) ES cells were injected subcutaneously into nude mice, teratocarcinoma-like tumors developed from ES cells. However, only tumors d erived from Parp(-/-) ES cells showed trophoblast giant cells (TGCs) contai ning single or multiple megalo-nuclei. These TGCs are located in a large bl ood-lake like hemorrhage. This example suggests that Parp is not essential for tumor formation, however, it is involved in trophoblastic cell differen tiation and could consequently affect tumor phenotype. (C) 2001 Elsevier Sc ience B.V. All rights reserved.