A dysfunctional movement protein of Tobacco mosaic virus interferes with targeting of wild-type movement protein to microtubules

Citation
G. Kotlizky et al., A dysfunctional movement protein of Tobacco mosaic virus interferes with targeting of wild-type movement protein to microtubules, MOL PL MICR, 14(7), 2001, pp. 895-904
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN journal
0894-0282 → ACNP
Volume
14
Issue
7
Year of publication
2001
Pages
895 - 904
Database
ISI
SICI code
0894-0282(200107)14:7<895:ADMPOT>2.0.ZU;2-P
Abstract
The Tobacco mosaic virus (TMV) movement protein (MPTMV) mediates cell-to-ce ll viral trafficking by altering properties of the plasmodesmata (Pd) in in fected cells. During the infection cycle, MPTMV becomes transiently associa ted with endomembranes, microfilaments, and microtubules (MT). It has been shown that the cell-to-cell spread of TMV is reduced in plants expressing t he dysfunctional MP mutant MPNT-1. To expand our understanding of the MP fu nction, we analyzed events occurring during the intracellular and intercell ular targeting of MPTMV and MPNT-1 when expressed as a fusion protein to gr een fluorescent protein (GFP), either by biolistic bombardment in a viral-f ree system or from a recombinant virus, The accumulation of MPTMV:GFP, when expressed in a viral-free system, is similar to MPTMV:GFP in TMV-infected tissues. Pd localization and cell-to-cell spread are late events, occurring only after accumulation of MP:GFP in aggregate bodies and on MT in the tar get cell. MPNT-1:GFP localizes to MT but does not target to Pd nor does it move cell to cell. The spread of transiently expressed MPTMV:GFP in leaves of transgenic plants that produce MPNT-1 is reduced, and targeting of the M PTMV:GFP to the cytoskeleton is inhibited. Although MPTMV:GFP targets to th e Pd in these plants, it is partially impaired for movement. It has been su ggested that MPNT-1 interferes with host-dependent processes that occur dur ing the intracellular targeting program that makes MP movement competent.