Evaluation of quantitative measurements of hepatitis C virus RNA to predict sustained response to interferon by genotype

Citation
K. Chayama et al., Evaluation of quantitative measurements of hepatitis C virus RNA to predict sustained response to interferon by genotype, J VIROL MET, 95(1-2), 2001, pp. 33-45
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGICAL METHODS
ISSN journal
0166-0934 → ACNP
Volume
95
Issue
1-2
Year of publication
2001
Pages
33 - 45
Database
ISI
SICI code
0166-0934(200106)95:1-2<33:EOQMOH>2.0.ZU;2-J
Abstract
Hepatitis C virus (HCV) virus load is one of the most important predictive factors for the outcome of interferon (IFN) therapy. Recent technological a dvances have allowed a more precise measurement of HCV load. However, the e xact cutoff values that could be used to predict the outcome of IFN have no t been established for each assay. Five recent quantitative assays were eva luated for the measurement of HCV (Amplicor monitor ver 1.0. Amplicor monit or ver 2.0 (GT), Amplicor monitor ver 2.0 (Cobas), Quantiplex branched DNA amplification (bDNA) ver 2.0 and HCV core protein level by enzyme immunosor bent assay) in 209 consecutive patients with chronic hepatitis C, who recei ved IFN therapy. The results of the two second generation Amplicor monitor tests (GT and Cobas) showed the best correlation (r = 0.930), but the other tests also showed relatively good correlations (r = 0.646-0.925). Each met hod predicted the effect of IFN with comparable predictive efficacy, rangin g from 77.0 to 80.8%. Receiver operating characteristic (ROC) curve analysi s showed that Amplicor monitor ver 2.0 and bDNA ver 2.0 are superior in pre dicting the response in genotype 2a. The best cutoff value for predicting t he response to IFN was different by genotype, which should be considered in selecting candidates for IFN treatment. (C) 2001 Elsevier Science B.V. All rights reserved.