Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation

Citation
S. Persad et al., Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation, J CELL BIOL, 153(6), 2001, pp. 1161-1173
Citations number
55
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
153
Issue
6
Year of publication
2001
Pages
1161 - 1173
Database
ISI
SICI code
0021-9525(20010611)153:6<1161:TSPINA>2.0.ZU;2-G
Abstract
beta -Catenin is a protein that plays a role in intercellular adhesion as w ell as in the regulation of gene expression. The latter role of beta -caten in is associated with its oncogenic properties due to the loss of expressio n or inactivation of the tumor suppressor adenomatous polyposis coli (APC) or mutations in beta -catenin itself. We now demonstrate that another tumor suppressor, PTEN, is also involved in the regulation of nuclear beta -cate nin accumulation and T cell factor (TCF) transcriptional activation in an A PC-independent manner. We show that nuclear beta -catenin expression is con stitutively elevated in PTEN null cells and this elevated expression is red uced upon reexpression of PTEN. TCF promoter/luciferase reporter assays and gel mobility shift analysis demonstrate that PTEN also suppresses TCF tran scriptional activity. Furthermore, the constitutively elevated expression o f cyclin D1, a beta -catenin/TCF-regulated gene, is also suppressed upon re expression of PTEN. Mechanistically, PTEN increases the phosphorylation of beta -catenin and enhances its rate of degradation. We define a pathway tha t involves mainly integrin-linked kinase and glycogen synthase kinase 3 in the PTEN-dependent regulation of beta -catenin stability, nuclear beta -cat enin expression, and transcriptional activity. Our data indicate that beta -catenin/TCF-mediated gene transcription is regulated by PTEN, and this may represent a key mechanism by which PTEN suppresses tumor progression.