Modification of phosphatidylcholine liposomes with poly(ethylene oxide)-substituted lipids

Citation
N. Morone et al., Modification of phosphatidylcholine liposomes with poly(ethylene oxide)-substituted lipids, J BIOACT C, 16(3), 2001, pp. 167-193
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
ISSN journal
0883-9115 → ACNP
Volume
16
Issue
3
Year of publication
2001
Pages
167 - 193
Database
ISI
SICI code
0883-9115(200105)16:3<167:MOPLWP>2.0.ZU;2-B
Abstract
Modification of egg phosphatidylcholine (eggPC) and dimyristoylphosphatidyl choline liposomes with poly(ethylene oxide)-substituted lipids [PEO-lipid( m,n ); m and n mean, respectively, the chain length of alkyl group and the number of PEO segments]. Morphologies of these supermolecules were investig ated by means of laser dynamic light scattering and transmission electron m icroscopy. Reconstitution of the PEO-lipid to eggPC liposome was possible b elow 40-50 mol% of total lipids for PEO- lipid(12,5), and 30-40 mol% for PE O-lipid(12,13) and PEO-lipid(12,31). Effective encapsulation of FITC-dextra n (1.96 kDa) into the liposome was obtained using the eggPC liposome modifi ed with PEO-lipid. This PEO-modified liposome showed sufficient barrier fun ction to water-soluble polymers such as dextran. Through various studies, i t was found that PEO-lipid(12,13) is relatively compatible for modifying co nventional eggPC liposome.