Analysis of expressed sequence tags from a cDNA library of somatic nucleartransfer-derived cloned bovine whole foetus

Citation
Y. Taniguchi et al., Analysis of expressed sequence tags from a cDNA library of somatic nucleartransfer-derived cloned bovine whole foetus, ANIM GENET, 32(1), 2001, pp. 1-6
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences","Molecular Biology & Genetics
Journal title
ANIMAL GENETICS
ISSN journal
0268-9146 → ACNP
Volume
32
Issue
1
Year of publication
2001
Pages
1 - 6
Database
ISI
SICI code
0268-9146(200102)32:1<1:AOESTF>2.0.ZU;2-1
Abstract
The expression profile of genes in specific tissues is studied through anal ysing expressed sequence tags (ESTs) and provides useful information for ch aracterizing gene function and tissue physiology. Analysis of ESTs is achie ved by partial sequencing and characterization of clones isolated randomly from cDNA libraries. In the present study, we analysed the genes expressed in the somatic nuclear transfer-derived cloned bovine foetus in the early p eriod of foetal development. To this aim, we constructed a directionally cl oned cDNA library from somatic nuclear transfer-derived cloned 60 day-old w hole foetus of cattle and sequenced 3' end of 510 randomly isolated clones. By BLASTN analysis, we identified 403 unique clones: 186 showed homology t o previously identified genes, 123 matched uncharacterized ESTs and 94 show ed no significant matches to sequences already present in DNA databases. An alysis of these cDNA clones revealed that this library contained a variety of functional genes, while foetuin, insulin-like growth factor 2, collagen type I alphaI and maternal G10 transcript genes were the most abundant tran scripts. Our study allowed the establishment of a first list of genes expre ssed in bovine whole foetus. In future, the list of genes might help facili tate the understanding of physiology of foetal development in somatic nucle ar transfer-derived cloned bovine foetus.