Dissociation between T helper type 1 and type 2 differentiation and cytokine production in tumor-infiltrating lymphocytes in patients with lung cancer

Citation
N. Ito et al., Dissociation between T helper type 1 and type 2 differentiation and cytokine production in tumor-infiltrating lymphocytes in patients with lung cancer, SURG TODAY, 31(5), 2001, pp. 390-394
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
SURGERY TODAY
ISSN journal
0941-1291 → ACNP
Volume
31
Issue
5
Year of publication
2001
Pages
390 - 394
Database
ISI
SICI code
0941-1291(2001)31:5<390:DBTHT1>2.0.ZU;2-C
Abstract
We recently reported the balance of T helper type 1 (Th1) cells and type 2 (Th2) cells in patients with lung carcinomas. This study was conducted to i nvestigate their activity and role in tumors, which remain unclear. We dete rmined the population of lymphocytes with intracellular interferon (IFN)-ga mma or interleukin (IL)-4 by flow cytometry, and investigated cytokine prod uction using enzyme-linked immunosorbent assay (ELISA) in 22 patients with non-small cell lung cancer. The IFN-gamma -positive subset showed a signifi cant increase in the number of tumor-infiltrating lymphocytes (TIL) compare d with the peripheral blood lymphocytes (PBL) (PBL, 13.8% +/- 1.5%; TIL, 34 .3% +/- 3.4%; P < 0.001), and the IL-4 positive subset showed reverse resul ts (PBL, 3.7% +/- 0.6%; TIL, 2.1% +/- 0.3%; P = 0.037), However. TIL did no t produce more IFN-gamma than PBL. The results of intracellular IFN-gamma a nalysis and the production of IFN-gamma in PBL and TIL were significantly c orrelated (PBL: n = 22, r = 0.50, P = 0.025; TIL: n = 22, r = 0.44, P = 0.0 22). The dissociation between Th1 differentiation and IFN-gamma production in TIL was one of the host factors influencing the immune anergy against tu mors.