Renal morphology in connexin43 knockout mice

Citation
Dm. Silverstein et al., Renal morphology in connexin43 knockout mice, PED NEPHROL, 16(6), 2001, pp. 467-471
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pediatrics
Journal title
PEDIATRIC NEPHROLOGY
ISSN journal
0931-041X → ACNP
Volume
16
Issue
6
Year of publication
2001
Pages
467 - 471
Database
ISI
SICI code
0931-041X(200106)16:6<467:RMICKM>2.0.ZU;2-P
Abstract
Connexins (Cx) are a family of proteins that constitute the intercellular m embrane channels of gap junctions. These junctions permit intercellular mov ement of ions and other molecules between cells. a property vital to organo genesis. Cx43 is a member of the family of channel-forming proteins that ar e essential for cell-cell communication of developmental signals. Studies d emonstrate that Cx43 is observed in mesenchymal cells of 12-day gestation m ouse kidney, a crucial period of renal development. In order to study the s ignificance of Cx43 on renal developmental morphology, we evaluated the kid neys of embryos lacking the gene encoding for Cx43. Polymerase chain reacti on (PCR) from tail specimens identified wild-type (WT), heterozygote (HT) a nd knockout (KO) progeny. In situ RT-PCR displayed abundant Cx43 staining i n glomeruli, vasculature, and tubules in kidneys obtained from WT progeny. In contrast, Cx43 expression was completely absent in kidneys isolated from the KO. Renal histology in all three groups displayed no significant diffe rences. Renal size was similar and there was no evidence of dysplasia or cy st formation in the KO. Our results indicate that absence of Cx43, heretofo re considered essential for renal development, does not affect early renal morphological development.