The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment

Citation
P. Marra et al., The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment, NAT CELL BI, 3(12), 2001, pp. 1101-1113
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
NATURE CELL BIOLOGY
ISSN journal
1465-7392 → ACNP
Volume
3
Issue
12
Year of publication
2001
Pages
1101 - 1113
Database
ISI
SICI code
1465-7392(200112)3:12<1101:TGAGGP>2.0.ZU;2-A
Abstract
Integrating the pleomorphic membranes of the intermediate compartment (IC) into the array of Golgi cisternae is a crucial step in membrane transport, but it is poorly understood. To gain insight into this step, we investigate d the dynamics by which cis-Golgi matrix proteins such as GM130 and GRASP65 associate with, and incorporate, incoming IC elements. We found that GM130 and GRASP65 cycle via membranous tubules between the Golgi complex and a c onstellation of mobile structures that we call late IC stations. These stat ions are intermediate between the IC and the cis-Golgi in terms of composit ion, and they receive cargo from earlier IC elements and deliver it to the Golgi complex. Late IC elements are transient in nature and sensitive to fi xatives; they are seen in only a fraction of fixed cells, whereas they are always visible in living cells. Finally, late IC stations undergo homotypic fusion and establish tubular connections between themselves and the Golgi. Overall, these features indicate that late IC stations mediate the transit ion between IC elements and the cis-Golgi face.