Neurotransmitter dopamine applied in electrochemical determination of aluminum in drinking waters and biological samples

Citation
Fp. Zhang et al., Neurotransmitter dopamine applied in electrochemical determination of aluminum in drinking waters and biological samples, J INORG BIO, 87(1-2), 2001, pp. 105-113
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics","Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF INORGANIC BIOCHEMISTRY
ISSN journal
0162-0134 → ACNP
Volume
87
Issue
1-2
Year of publication
2001
Pages
105 - 113
Database
ISI
SICI code
0162-0134(200111)87:1-2<105:NDAIED>2.0.ZU;2-X
Abstract
It was demonstrated that the decrease of the differential pulse voltammetri c (DPV) anodic peak current of dopamine (3,4-dihydroxyphenylethylamine, DA) was linear with the increase of aluminum (AI) concentration. Under optimum experimental conditions (pH 4.6, 1.2 x 10(-3) M DA, and 0.04 M NaAc-HAc bu ffer solution), the linear range is 4.0 x 10(-7)-8.0 x 10(-5) M, the detect ion limit is 1.4 x 10(-7) M, and the relative standard deviation for 4 x 10 (-5) M Al-III is 3.5% (n=8). Many foreign species, especially some low-mole cule-weight biological molecules, were chosen for interference testing. The proposed method was applied to the determination of Al in biological sampl es such as synthetic renal dialysate, Ringer's solution, human blood, cereb rospinal fluid of a patient, and urine of a diabetic patient. The correspon ding recoveries were generally between 95 and 105%. The basic principle of the method was determined by examining Al complexed with DA. This results i n the blockage of the electroactive sites on DA, followed eventually by the reduction of the electrochemical response of DA. This result was verified by examining the behavior of DA, both in the presence and absence of Al, us ing electrochemical, UV-Vis, Raman, and C-13 NMR spectroscopic methods. (C) 2001 Elsevier Science BY All rights reserved.