Exclusion of CD43 from the immunological synapse is mediated by phosphorylation-regulated relocation of the cytoskeletal adaptor moesin

Citation
J. Delon et al., Exclusion of CD43 from the immunological synapse is mediated by phosphorylation-regulated relocation of the cytoskeletal adaptor moesin, IMMUNITY, 15(5), 2001, pp. 691-701
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
IMMUNITY
ISSN journal
1074-7613 → ACNP
Volume
15
Issue
5
Year of publication
2001
Pages
691 - 701
Database
ISI
SICI code
1074-7613(200111)15:5<691:EOCFTI>2.0.ZU;2-Q
Abstract
Formation of the immunological synapse requires TOR signal-dependent protei n redistribution. However, the specific molecular mechanisms controlling pr otein relocation are not well defined. Moesin is a widely expressed phospho -protein that links many transmembrane molecules to the cortical actin cyto skeleton. Here, we demonstrate that TCR-induced exclusion of the large sial oprotein CD43 from the synapse is an active event mediated by its reversibl e binding to moesin. Our results also reveal that relocalization of moesin is associated with changes in the phosphorylation status of this cytoskelet al adaptor protein. Finally, these findings raise the possibility that the change in moesin localization resulting from TCR engagement modifies the ov erall topology of the lymphocyte membrane and facilitates molecular interac tions at the site of presenting cell contact.