A rapid screening method for a single nucleotide polymorphism (SNP) in thehuman MOR gene

Citation
S. Grosch et al., A rapid screening method for a single nucleotide polymorphism (SNP) in thehuman MOR gene, BR J CL PH, 52(6), 2001, pp. 711-714
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology,"Pharmacology & Toxicology
Journal title
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
ISSN journal
0306-5251 → ACNP
Volume
52
Issue
6
Year of publication
2001
Pages
711 - 714
Database
ISI
SICI code
0306-5251(200112)52:6<711:ARSMFA>2.0.ZU;2-P
Abstract
Aims Genetic association Studies have suggested chat the single nucleotide polymorphism (SNP) at position 118 of the human mu -opioid receptor (MOR) g ene could be a potential risk Factor For drug treatment variability in pati ents. Therefore, we wanted to develop a fast and reliable detection method for this SNP which is applicable in a clinical setting. Methods To detect the polymorphism at position A118-->G in the human MOR, g ene we used the fluorescence resonance energy transfer (FRET)-PCR technique with subsequent melting curve analysis. Results The polymorphism at position A118-->G in the human MOR gene could b e clearly discriminated with melting peak temperatures of 69.8 degrees C an d 63.8 degrees C, corresponding to the wild type and mutated MOR allele, re spectively. The results from FRET-PCR were validated by sequencing and rest riction-fragment length polymorphism (RFLP). Screening of blood samples fro m 100 subjects showed an allelic distribution for the human MOR alleles of 79% homozygous wild type). 20% (heterozygous) and 0.9% (homozygous mutated) . Conclusions The FRET-PCR protocol for detection of the human MOR gene polym orphism at position 118 offers a rapid and reliable method which could be u sed for population screening of this and other genes.