Purification and characterization of a salicylate hydroxylase involved in 1-hydroxy-2-naphthoic acid hydroxylation from the naphthalene and phenanthrene-degrading bacterial strain Pseudomonas putida BS202-P1

Citation
Nv. Balashova et al., Purification and characterization of a salicylate hydroxylase involved in 1-hydroxy-2-naphthoic acid hydroxylation from the naphthalene and phenanthrene-degrading bacterial strain Pseudomonas putida BS202-P1, BIODEGRADAT, 12(3), 2001, pp. 179-188
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIODEGRADATION
ISSN journal
0923-9820 → ACNP
Volume
12
Issue
3
Year of publication
2001
Pages
179 - 188
Database
ISI
SICI code
0923-9820(2001)12:3<179:PACOAS>2.0.ZU;2-5
Abstract
1-Hydroxy-2-naphthoate is formed as an intermediate in the bacterial degrad ation of phenanthrene. A monooxygenase which catalyzed the oxidation of 1-h ydroxy-2-naphthoate to 1,2-dihydroxynaphthalene was purified from the phena nthrene- and naphthalene-degrading Pseudomonas putida strain BS202-P1. The purified protein had a molecular weight of 45 kDa and required NAD(P)H and FAD as cofactors. The purified enzyme also catalysed the oxidation of salic ylate and various substituted salicylates. The comparison of the K-m and V- max values for 1-hydroxy-2-naphthoate and salicylate demonstrated a higher catalytic efficiency of the enzyme for salicylate as a substrate. A signifi cant substrate-inhibition was detected with higher concentrations of 1-hydr oxy-2-naphthoate. The aminoterminal amino acid sequence of the purified enz yme showed significant homologies to salicylate 1-monooxygenases from other Gram negative bacteria. It was therefore concluded that during the degrada tion of phenanthrene the conversion of 1-hydroxy-2-naphthoate to 1,2-dihydr oxynaphthalene is catalysed by a salicylate 1-monooxygenase. Together with previous studies, this suggested that the enzymes of the naphthalene pathwa y are sufficient to catalyse also the mineralization of phenanthrene.