Protein degradation during anaerobic wastewater treatment: derivation of stoichiometry

Citation
Ir. Ramsay et Pc. Pullammanappallil, Protein degradation during anaerobic wastewater treatment: derivation of stoichiometry, BIODEGRADAT, 12(4), 2001, pp. 247-257
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIODEGRADATION
ISSN journal
0923-9820 → ACNP
Volume
12
Issue
4
Year of publication
2001
Pages
247 - 257
Database
ISI
SICI code
0923-9820(2001)12:4<247:PDDAWT>2.0.ZU;2-L
Abstract
The stoichiometry of reactions that describe protein degradation in anaerob ic treatment systems were investigated. A methodology was developed to desc ribe protein degradation to organic acids using a single reaction step. The reactions for individual amino acid fermentation and their mediating organ isms were reviewed. The dominant fermentation pathways were selected based on a number of assumptions. Using the amino acid content of a model protein , it was then possible to determine stoichiometric coefficients for each ma jor organic acid product in the overall degradation of the protein. The the oretical coefficients were then compared to those determined from two exper imental runs on a continuously-fed, well-mixed, laboratory-scale anaerobic wastewater treatment system. In general, the coefficients compared well thu s validating the use of a single reaction step for the overall catabolic re action of protein degradation to organic acids. Furthermore, even when the protein concentration in feed or the feed flow rate was doubled, the amino acid fermentation pathways were found to occur predominantly by only one pa thway. Although the choice of Stickland reactions over uncoupled degradatio n provided good comparisons, an electron balance showed that only about 40% of the amino acids could have proceeded coupled to other amino acid reacti ons. Uncoupled degradation of the remaining amino acids must have relied on the uptake of hydrogen produced from these reactions by hydrogen-consuming methane bacteria.