Brucella lipopolysaccharides induce cyclooxygenase-2 expression in monocytic cells

Citation
L. Lopez-urrutia et al., Brucella lipopolysaccharides induce cyclooxygenase-2 expression in monocytic cells, BIOC BIOP R, 289(2), 2001, pp. 372-375
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
289
Issue
2
Year of publication
2001
Pages
372 - 375
Database
ISI
SICI code
0006-291X(20011130)289:2<372:BLICEI>2.0.ZU;2-N
Abstract
Human brucellosis is characterized by the presence of both acute inflammato ry episodes and chronic inflammation with granuloma formation. On this basi s, the proinflammatory effects of smooth lipopolysaccharide of Brucella (S- LPS) were addressed and compared to those of LPS from Escherichia coli. For this purpose, the induction of cyclooxygenase-2 (COX-2), the production of the chemokine monocyte chemoattractant protein-1 (MCP-1), and the activati on of the nuclear factor kappaB (NF-kappaB) were studied. S-LPS was found t o induce both COX-2 expression and MCP-1 production; however, the potency o f E. coli LPS exceeded that of Brucella S-LPS by some orders of magnitude. However, at concentrations above 1 mug/ml, all of the LPS produced comparab le effects, including their ability to activate the NF-kappaB system. These observations help explain the inflammatory events associated with Brucella infection and the ability of Brucella to produce monocyte recruitment and granuloma formation. (C) 2001 Elsevier Science.