Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production

Citation
T. Matsumoto et al., Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production, APPL MICR B, 57(4), 2001, pp. 515-520
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN journal
0175-7598 → ACNP
Volume
57
Issue
4
Year of publication
2001
Pages
515 - 520
Database
ISI
SICI code
0175-7598(200111)57:4<515:YWBCBI>2.0.ZU;2-M
Abstract
Yeast whole-cell biocatalysts for lipase-catalyzed reactions were construct ed by intracellularly overproducing Rhizopus oryzae lipase (ROL) in Sacchar omyces cerevisiae MT8-1. The gene encoding lipase from R. oryzae IFO4697 wa s cloned, and intracellular overproduction systems of a recombinant ROL wit h a pro-sequence (rProROL) were constructed. When rProROL from R. oryzae IF O4697 was produced under the control of the 5'-upstream region of the isoci trate lyase gene of Candida tropicalis (UPR-ICL) at 30 degreesC for 98 h by two-stage cultivation using SDC medium (SD medium with 2% casamino acids) containing 2.0% and 0.5% glucose, intracellular lipase activity reached lev els up to 474.5 IU/l. These whole-cell biocatalysts were permeabilized by a ir-drying and used for the synthesis of methyl esters (MEs), a potential bi odiesel fuel, from plant oil and methanol in a solvent-free and water-conta ining system. The ME content in the reaction mixture was71 wt% after a 165- h reaction at 37 degreesC with stepwise addition of methanol. These results indicate that an efficient whole-cell biocatalyst can be prepared by intra cellular overproduction of lipase in yeast cells and their permeabilization .