Generation of dendritic cells from leukaemia cells of a patient with acutepromyelocytic leukaemia by culture with GM-CSF, IL-4 and TNF-alpha

Narita, M Takahashi, M Liu, AC Ayres, F Satoh, N Abe, T Nikkuni, K Furukawa, T Toba, K Aizawa, Y

M. Narita et al., Generation of dendritic cells from leukaemia cells of a patient with acutepromyelocytic leukaemia by culture with GM-CSF, IL-4 and TNF-alpha, ACT HAEMAT, 106(3), 2001, pp. 89-94

15

INGLESE

Article

Hematology,"Cardiovascular & Hematology Research

ACTA HAEMATOLOGICA

0001-5792 → ACNP

106

3

2001

89 - 94

ISI

0001-5792(2001)106:3<89:GODCFL>2.0.ZU;2-K

Dendritic cells are potent antigen-presenting cells derived from CD34+ haem opoietic stem cells. Dendritic cells have been reported to be generated fro m cells in granulocytic lineage as well as monocytes, blood dendritic cell precursors and lymphoid progenitors. In order to explore the differentiatio n pathway of dendritic cells from granulocytic cells and the applicability of leukaemia-derived dendritic cells for anti-leukaemic immunotherapy in ac ute leukaemia of granulocytic origin, we tried to generate dendritic cells from leukaemia cells of a patient with acute promyelocytic leukaemia (APL). Leukaemia cells were cultured with GM-CSF, IL-4 and TNF-alpha for 10 days. Azurophilic granule-containing cells with marked cytoplasmic projections w ere generated in the culture. FACS analysis of these cultured cells reveale d the generation of CD1a+, CD83+, CD80+, CD86+, CD40+ and HLA-DR+ cells. Th e leukaemic origin of these dendritic-like cells was demonstrated by in sit u hybridization of magnetic-bead-sorted CD1a+ dendritic cells using the DNA probes of t(15;17). Cells generated by culturing leukaemia cells were demo nstrated to have a potent antigen-presenting function in allogeneic mixed l eucocyte cultures. These findings show the plausibility of the previously r eported pathway of dendritic cell maturation through granulocytic cells and suggest the possibility of anti-leukaemic immunotherapy using leukaemia-de rived dendritic cells even in patients with acute promyelocytic leukaemia. Copyright (C) 2001 S. Karger AG, Basel.