Dendritic cells are potent antigen-presenting cells derived from CD34+ haem
opoietic stem cells. Dendritic cells have been reported to be generated fro
m cells in granulocytic lineage as well as monocytes, blood dendritic cell
precursors and lymphoid progenitors. In order to explore the differentiatio
n pathway of dendritic cells from granulocytic cells and the applicability
of leukaemia-derived dendritic cells for anti-leukaemic immunotherapy in ac
ute leukaemia of granulocytic origin, we tried to generate dendritic cells
from leukaemia cells of a patient with acute promyelocytic leukaemia (APL).
Leukaemia cells were cultured with GM-CSF, IL-4 and TNF-alpha for 10 days.
Azurophilic granule-containing cells with marked cytoplasmic projections w
ere generated in the culture. FACS analysis of these cultured cells reveale
d the generation of CD1a+, CD83+, CD80+, CD86+, CD40+ and HLA-DR+ cells. Th
e leukaemic origin of these dendritic-like cells was demonstrated by in sit
u hybridization of magnetic-bead-sorted CD1a+ dendritic cells using the DNA
probes of t(15;17). Cells generated by culturing leukaemia cells were demo
nstrated to have a potent antigen-presenting function in allogeneic mixed l
eucocyte cultures. These findings show the plausibility of the previously r
eported pathway of dendritic cell maturation through granulocytic cells and
suggest the possibility of anti-leukaemic immunotherapy using leukaemia-de
rived dendritic cells even in patients with acute promyelocytic leukaemia.
Copyright (C) 2001 S. Karger AG, Basel.