Absorption spectrum of the green fluorescent protein chromophore anion in vacuo - art. no. 2281092

Citation
Sb. Nielsen et al., Absorption spectrum of the green fluorescent protein chromophore anion in vacuo - art. no. 2281092, PHYS REV L, 8722(22), 2001, pp. 8102
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physics
Journal title
PHYSICAL REVIEW LETTERS
ISSN journal
0031-9007 → ACNP
Volume
8722
Issue
22
Year of publication
2001
Database
ISI
SICI code
0031-9007(20011126)8722:22<8102:ASOTGF>2.0.ZU;2-O
Abstract
A sensitive photoabsorption technique for studies of gas-phase biomolecules has been used at the ELISA electrostatic heavy-ion storage ring. We show t hat the anion form of the chromophore of the green fluorescent protein in v acuo has an absorption maximum at 479 nm, which coincides with one of the t wo absorption peaks of the protein. Its absorption characteristics are ther efore ascribed to intrinsic chemical properties of the chromophore. Evident ly, the special beta -can structure of the protein provides shielding of th e chromophore from the surroundings without significantly changing the elec tronic structure of the chromophore through interactions with amino acid si de chains.