Bacteriophage P2: recombination in the superinfection preprophage state and under replication control by phage P4

Citation
G. Bertani et G. Deho, Bacteriophage P2: recombination in the superinfection preprophage state and under replication control by phage P4, MOL GENET G, 266(3), 2001, pp. 406-416
Citations number
62
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR GENETICS AND GENOMICS
ISSN journal
1617-4615 → ACNP
Volume
266
Issue
3
Year of publication
2001
Pages
406 - 416
Database
ISI
SICI code
1617-4615(200111)266:3<406:BPRITS>2.0.ZU;2-D
Abstract
Genetic crosses (mixed infection, lytic cycle) with bacteriophage P2 are kn own to give extremely low recombination frequencies, and these are unaffect ed by the recA status of the host bacterium. We now show the following: (1) the satellite bacteriophage P4, which interacts with P2 in a number of way s, but is quite different from it in terms of DNA replication and its contr ol, is clearly dependent on the host recA(+) function for recombination, (2 ) a chimeric phage (Lindqvist's P2/P4 Hy19), in which P2 replication early genes have been replaced by those of P4, recombines in a recA(+)-dependent manner; (3) immunity-sensitive P2 phages, in mixed infections of P2-immune bacteria, and hence blocked in their replication, recombine in a recA(+)-de pendent manner; (4) an analysis of the distribution of exchanges based on a simple model confirms that in mixed infections of sensitive cells (where P 2 is actively multiplying) recombinational exchanges tend to be statistical ly clustered in a segment of the chromosome containing the origin of replic ation, and also shows that, under conditions in which P2 DNA replication is blocked, the distribution of exchanges correlates well with the physical d istances between markers on the P2 DNA.