New reversed-phase liquid chromatographic method to detect aflatoxins in food and feed with cyclodextrins as fluorescence enhancers added to the eluent

Citation
E. Chiavaro et al., New reversed-phase liquid chromatographic method to detect aflatoxins in food and feed with cyclodextrins as fluorescence enhancers added to the eluent, J CHROMAT A, 937(1-2), 2001, pp. 31-40
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
937
Issue
1-2
Year of publication
2001
Pages
31 - 40
Database
ISI
SICI code
0021-9673(200112)937:1-2<31:NRLCMT>2.0.ZU;2-I
Abstract
The effect of succynil-beta -cyclodextrin (beta -CD-Su), dimethyl-beta -cyc lodextrin (DIMEB) and beta -cyclodextrin (beta -CD) on the fluorescence of aflatoxins B-1, B-2, G(1), G(2) and M-1 (AFB(1), AFB(2), AFG(1), AFG(2) and AFM(1)) was studied: beta -CD-Su promoted the largest fluorescence enhance ment for AFB(1) and AFM(1) while DIMEB showed better results for AFG(1). On the basis of the fluorescence enhancement, a new RP-HPLC method for detect ing aflatoxins B-1, B-2, G(1), G(2) and M-1 was developed using cyclodextri ns directly dissolved in the LC eluent. Aflatoxins B-1, B-2, G(1) and G(2) were resolved using a MICRA NPS ODS-1 column using methanol-water as mobile phase to which 6x10(-3) M beta -CD-Su or beta -CD were added. Chromatograp hic responses of AFB(1) and AFG(1) achieved using beta -CD dissolved in the mobile phase were enhanced, respectively, 8 and 12 times, and 10 and 15 ti mes with beta -CD-Su. Detection limits lower than 0.3 mug/kg were achieved for all the four aflatoxins. Aflatoxin M-1 was analysed using a Spherisorb S3 ODS-2 Narrow Bore column and methanol-water as mobile phase with added 2 x10(-3) M beta -CD-Su. An area enhancement of 1.5 was detected for the toxi n and the detection Emit achieved under these analytical conditions was low er than 0.0005 mug/kg. Both methods were statistically validated showing a linear response for all the aflatoxins tested (R(2)greater than or equal to 0.99), and applied to the analysis of spiked and naturally contaminated foo d samples. (C) 2001 Elsevier Science B.V. All rights reserved.