NAPO as a novel marker for apoptosis

Citation
Bs. Sayan et al., NAPO as a novel marker for apoptosis, J CELL BIOL, 155(5), 2001, pp. 719-724
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
155
Issue
5
Year of publication
2001
Pages
719 - 724
Database
ISI
SICI code
0021-9525(20011126)155:5<719:NAANMF>2.0.ZU;2-B
Abstract
Apoptosis or programmed cell death plays a pivotal role in embryonic develo pment and maintenance of homeostasis. It is also involved in the etiology o f pathophysiological conditions such as cancer, neurodegenerative, autoimmu ne, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody-defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen i s maintained in quiescent and senescent cells, as well as in different phas es of the cell cycle, including mitosis. Thus, immunodetection of NAPO anti gen provides a specific, sensitive, and easy method for differential identi fication of apoptotic and nonapoptotic cells.