Correct spindle elongation at the metaphase/anaphase transition is an APC-dependent event in budding yeast

Citation
F. Severin et al., Correct spindle elongation at the metaphase/anaphase transition is an APC-dependent event in budding yeast, J CELL BIOL, 155(5), 2001, pp. 711-717
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
155
Issue
5
Year of publication
2001
Pages
711 - 717
Database
ISI
SICI code
0021-9525(20011126)155:5<711:CSEATM>2.0.ZU;2-K
Abstract
At the metaphase to anaphase transition, chromosome segregation is initiate d by the splitting of sister chromatids. Subsequently, spindles elongate, s eparating the sister chromosomes into two sets. Here, we investigate the ce ll cycle requirements for spindle elongation in budding yeast using mutants affecting sister chromatid cohesion or DNA replication. We show that separ ation of sister chromatids is not sufficient for proper spindle integrity d uring elongation. Rather, successful spindle elongation and stability requi re both sister chromatid separation and anaphase-promoting complex activati on. Spindle integrity during elongation is dependent on proteolysis of the securin Pds1 but not on the activity of the separase Esp1. Our data suggest that stabilization of the elongating spindle at the metaphase to anaphase transition involves Pds1-dependent targets other than Esp1.