Cloning and characterization of a novel human TEKTIN1 gene

Citation
M. Xu et al., Cloning and characterization of a novel human TEKTIN1 gene, INT J BIO C, 33(12), 2001, pp. 1172-1182
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
ISSN journal
1357-2725 → ACNP
Volume
33
Issue
12
Year of publication
2001
Pages
1172 - 1182
Database
ISI
SICI code
1357-2725(200112)33:12<1172:CACOAN>2.0.ZU;2-#
Abstract
Tektins comprise a family of filament-forming proteins that are known to be coassembled with tubulins to form ciliary and flagellar microtubules. A ne w member of the tektin gene family was cloned from the human fetal brain cD NA library. We hence named it the human TEKTIN1 gene. TEKTIN1 cDNA consists of 1375 bp and has a putative open reading frame encoding 418 amino acids. The predicted protein is 48.3 kDa in size, and its amino acid sequence is 82% identical to that of the mouse, rat. and dog. One conserved peptide RPN VELCRD was observed at position number 323-331 of the amino acid sequence, which is a prominent feature of tektins and is likely to represent a functi onally important protein domain. TEKTIN1 gene was mapped to the human chrom osome 17 by BLAST search, and at least eight exons were found. Northern blo t analysis indicated that TEKTIN1 was predominantly expressed in testis. By in-situ hybridization analysis, TEKTIN1 mRNA was localized to spermatocyte s and round spermatids in the seminiferous tubules of the mouse testis, ind icating that it may play a role in spermatogenesis. (C) 2001 Elsevier Scien ce Ltd. All rights reserved.