Tektins comprise a family of filament-forming proteins that are known to be
coassembled with tubulins to form ciliary and flagellar microtubules. A ne
w member of the tektin gene family was cloned from the human fetal brain cD
NA library. We hence named it the human TEKTIN1 gene. TEKTIN1 cDNA consists
of 1375 bp and has a putative open reading frame encoding 418 amino acids.
The predicted protein is 48.3 kDa in size, and its amino acid sequence is
82% identical to that of the mouse, rat. and dog. One conserved peptide RPN
VELCRD was observed at position number 323-331 of the amino acid sequence,
which is a prominent feature of tektins and is likely to represent a functi
onally important protein domain. TEKTIN1 gene was mapped to the human chrom
osome 17 by BLAST search, and at least eight exons were found. Northern blo
t analysis indicated that TEKTIN1 was predominantly expressed in testis. By
in-situ hybridization analysis, TEKTIN1 mRNA was localized to spermatocyte
s and round spermatids in the seminiferous tubules of the mouse testis, ind
icating that it may play a role in spermatogenesis. (C) 2001 Elsevier Scien
ce Ltd. All rights reserved.