A genome-wide survey of human thioredoxin and glutaredoxin family pseudogenes

Citation
G. Spyrou et al., A genome-wide survey of human thioredoxin and glutaredoxin family pseudogenes, HUM GENET, 109(4), 2001, pp. 429-439
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
HUMAN GENETICS
ISSN journal
0340-6717 → ACNP
Volume
109
Issue
4
Year of publication
2001
Pages
429 - 439
Database
ISI
SICI code
0340-6717(200110)109:4<429:AGSOHT>2.0.ZU;2-V
Abstract
The thioredoxin/glutaredoxin family consists of small heat-stable proteins that have a highly conserved CXXC active site and that participate in the r egulation of many redox reactions. We have searched the human genome sequen ce to find putative pseudogenes (non-functional copies of protein-coding ge nes) for all known members of this family. This survey has resulted in the identification of seven processed pseudogenes for human Trx1 and two more f or human Grx1. No evidence for the presence of processed pseudogenes has be en found for the remaining members of this family. In addition, we have bee n unable to detect any non-processed pseudogenes derived from any member of the family in the human genome. The seven thioredoxin pseudogenes can be d ivided into two groups: Trx1-psi2, -psi3, -psi4, -psi5 and -psi6 arose from the functional ancestor, whereas Trx1-psi1 and -psi7 originated from Trx1- psi2 and -psi6, respectively. In all cases, the pseudogenes originated afte r the human/rodent radiation as shown by phylogenetic analysis. This is als o the case for Grx1-psi1 and Grx1-psi2, which are placed between rodent and human sequences in the phylogenetic tree. Our study provides a molecular r ecord of the recent evolution of these two genes in the hominid lineage.