Detection of t(11;18) in MALT-type lymphoma with dual-color fluorescence in situ hybridization and reverse transcriptase-polymerase chain reaction analysis

Citation
Y. Kobayashi et al., Detection of t(11;18) in MALT-type lymphoma with dual-color fluorescence in situ hybridization and reverse transcriptase-polymerase chain reaction analysis, DIAGN MOL P, 10(4), 2001, pp. 207-213
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
DIAGNOSTIC MOLECULAR PATHOLOGY
ISSN journal
1052-9551 → ACNP
Volume
10
Issue
4
Year of publication
2001
Pages
207 - 213
Database
ISI
SICI code
1052-9551(200112)10:4<207:DOTIML>2.0.ZU;2-K
Abstract
t(11:18) is a recurrent chromosomal abnormality observed in mucosa-associat ed lymphoid tissue (MALT)-type lymphoma. AP12 and MLT genes have been impli cated. The authors devised a dual-color interphase fluorescence in situ hyb ridization (FISH) system to detect splitting of 11q22 and its fusion with 1 8q21. Subjects were 44 cases of extranodal lymphoma and cases of primary ma croglobulinemia. Whenever RNA was available, reverse transcriptase-polymera se chain reaction followed by sequence analysis was performed. Positive cas es by dual-color FISH analysis were restricted to MALT-type lymphoma and on e case of primary macroglobulinemia. Among 24 cases of MALT-type lymphoma. 14 (58%) (4 gastric, 5 pulmonary, 3 orbital, 1 salivary, and 1 thyroid lymp homas) had splitting of the 11q22 region probes and fusion of signals sugge sting the translocation of chromosome 11 and 18. Reverse transcriptase-poly merase chain reaction analysis showed the AP12/MLT gene fusion in 9 of 10 c ases. Sequence analyses showed three different modes of involvement of the MLT gene, whereas the breakpoint at AP12 was the same. Monoclonal component of serum immunoglobulin M was observed in 3 of 14 positive cases for the t ranslocation. Direct visualization using dual-color FISH on samples serves as a molecular tool for management of MALT-type lymphoma with API2/MLT gene fusion.