Effects of sepsis on mast cells in rat dura mater: influence of L-NAME andVIP

Citation
F. Tore et al., Effects of sepsis on mast cells in rat dura mater: influence of L-NAME andVIP, BR J PHARM, 134(7), 2001, pp. 1367-1374
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BRITISH JOURNAL OF PHARMACOLOGY
ISSN journal
0007-1188 → ACNP
Volume
134
Issue
7
Year of publication
2001
Pages
1367 - 1374
Database
ISI
SICI code
0007-1188(200112)134:7<1367:EOSOMC>2.0.ZU;2-B
Abstract
1 The influence of lipopolysaccharide (LPS)-induced sepsis on the various m ast cell phenotypes of rat dura mater were examined both by immunohistochem ical and biochemical methods. 2 Three different populations of mast cells were identified in control rats : connective tissue type mast cells (CTMC) which contain rat mast cell prot easel (RMCP1), histamine, serotonin and heparin, mucosal type mast cells (M MC) which contain RMCP2, histamine and serotonin, and intermediate type whi ch contains both RMCP1 and RMCP2 and probably various proportions of amines and heparin. 3 LPS (25 mg kg(-1) i.p.) caused changes in the proportions of the various types of mast cells. The number of MMC and intermediate type mast cells sig nificantly increased and the number of mast cells immunopositive for both h eparin and serotonin significantly decreased. Biochemical analysis showed t hat the histamine concentration of dura increased while its serotonin conce ntration decreased. 4 While vasoactive intestinal peptide (VIP) (25 ng kg(-1) i.p.) appears to potentiate LPS effects on dura mater mast cells, non-selective inhibition o f nitric oxide (NO) synthase by Ng-nitro-L-arginine methyl ester (L-NAME) ( 30 mg kg(-1) i.p.) did not influence sepsis-induced mast cell changes. 5 These findings suggest that mast cells of dura mater may play a role in b rain protection during sepsis.