Neural circuitry of the kidney: NO-containing neurons

Citation
Ml. Weiss et al., Neural circuitry of the kidney: NO-containing neurons, BRAIN RES, 919(2), 2001, pp. 269-282
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
0006-8993 → ACNP
Volume
919
Issue
2
Year of publication
2001
Pages
269 - 282
Database
ISI
SICI code
0006-8993(20011123)919:2<269:NCOTKN>2.0.ZU;2-A
Abstract
The neurons synthesizing nitric oxide (NO) that are part of the renal sympa thetic pathways were located by double-staining for the neuronal isoform of nitric oxide synthase (nNOS) using immunocytochemistry to identify NO-synt hesizing neurons and transneuronal tracing following infection of the left kidney with pseudorabies virus (PRV). Following kidney injection with PRV, the animals survived 4-day post-inoculation prior to sacrifice and tissue p rocessing. PRV-infected neurons that double-stained for nNOS were found in the paraventricular hypothalamic nucleus (PVN), the raphe obscurus nucleus (ROb), the ventromedial medulla (VMM), the rostral ventrolateral medulla (r VLM) and the A5 cell group. In the thoracolumbar spinal cord, nNOS neurons co-localized with PRV-infected cells in the dorsal horn in laminae I, III-V ipsilateral to the injected kidney and in lamina X, the intermediolateral cell column, the lateral funiculus, the intercalated nucleus and the centra l autonomic area. We conclude that NO synthesizing cells may significantly affect renal autonomic pathways in the rat by interacting with the renal se nsory and sympathomotor circuitry at multiple sites. (C) 2001 Elsevier Scie nce B.V. All rights reserved.