EVIDENCE FOR PROCTOLIN-LIKE AND RFAMIDE-LIKE NEUROPEPTIDES ASSOCIATEDWITH THE HINDGUT OF THE CRAYFISH PROCAMBARUS-CLARKII

Citation
Aj. Mercier et al., EVIDENCE FOR PROCTOLIN-LIKE AND RFAMIDE-LIKE NEUROPEPTIDES ASSOCIATEDWITH THE HINDGUT OF THE CRAYFISH PROCAMBARUS-CLARKII, Canadian journal of zoology, 75(8), 1997, pp. 1208-1225
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Zoology
Journal title
ISSN journal
0008-4301
Volume
75
Issue
8
Year of publication
1997
Pages
1208 - 1225
Database
ISI
SICI code
0008-4301(1997)75:8<1208:EFPARN>2.0.ZU;2-K
Abstract
Immunohistochemical staining revealed proctolin-like immunoreactivity in nerve endings associated with the hindgut of the crayfish Procambar us clarkii. Proctolin-like bioactivity, detected using both locust ovi ducts and crayfish hindguts for bioassays, co-eluted with authentic pr octolin through five consecutive reversed-phase high-performance liqui d chromatography (RP-HPLC) systems. This strongly suggests that procto lin (or a peptide very similar to it) is contained in nerve endings on the crayfish hindgut. RFamide-like immunoreactivity (RFLI) was extrac ted from the hindguts and intestinal nerves of crayfish and separated using RP-HPLC. Initial separation on a C18 column gave a broad peak of RFLI, and these fractions were bioactive on the crayfish hindgut. Sub sequently, RFLI was separated on two additional RP-HPLC systems. The p redominant peak could be distinguished from FMRFamide and several know n FMRFamide-like peptides on the basis of elution times. Partial seque nce analysis indicated the presence of a decapeptide having some seque nce homology with leucomyosuppressin and SchistoFLRFamide. These resul ts support earlier evidence that extended RFamide peptides may functio n as neurotransmitters or neuromodulators on the crayfish hindgut, and suggest a similar role for proctolin or a closely related peptide.