Molecular analysis of medicinally-used Chinese and Japanese Curcuma based on 18S rRNA gene and trnK gene sequences

Citation
H. Cao et al., Molecular analysis of medicinally-used Chinese and Japanese Curcuma based on 18S rRNA gene and trnK gene sequences, BIOL PHAR B, 24(12), 2001, pp. 1389-1394
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
12
Year of publication
2001
Pages
1389 - 1394
Database
ISI
SICI code
0918-6158(200112)24:12<1389:MAOMCA>2.0.ZU;2-D
Abstract
Curcuma drugs have been used discriminatingly for invigorating blood circul ation, promoting digestion, and as a cholagogic in China. However, there is confusion about the drug's botanical origins and clinical uses because of morphological similarity of Curcuma plants and drugs. In order to develop a n ultimate identification, molecular analysis based on 18S rRNA gene and tr nK gene sequences were performed on 6 Curcuma species used medicinally in C hina and Japan. The 18S rRNA gene sequences were found to be of 1810 bps in length. In comparison with the common sequence of C. longa, C. phaeocaulis , C. wenyujin and C. aromatica, that of C kwangsiensis had one base substit ution, and the same base difference was observed between the Chinese and th e Japanese populations of C. zedoaria. The trnK gene sequences were found t o span 2698-2705 bps. There were base substitutions, small deletions or ins ertions at some sites between the trnK coding region and matK region among each species. Based on the base substitutions, C zedoaria and C. kwangsiens is specimens were divided into two groups, respectively. An identical seque nce was detected in C phaeocaulis and in the Chinese population of C. zedoa ria, as well as in the Japanese population of C. zedoaria and in one group of C. kwangsiensis with a purple-colored band in leaves. New taxonomic info rmation to be used for authenticating Curcuma drugs was obtained.