The effects of lidocaine on superoxide production and p47 phox translocation in opsonized zymosan-activated neutrophils

Citation
K. Arakawa et al., The effects of lidocaine on superoxide production and p47 phox translocation in opsonized zymosan-activated neutrophils, ANESTH ANAL, 93(6), 2001, pp. 1501-1506
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Journal title
ANESTHESIA AND ANALGESIA
ISSN journal
0003-2999 → ACNP
Volume
93
Issue
6
Year of publication
2001
Pages
1501 - 1506
Database
ISI
SICI code
0003-2999(200112)93:6<1501:TEOLOS>2.0.ZU;2-5
Abstract
Superoxide is generated by reduced nicotinamide adenine dinucleotide phosph ate (NADPH) oxidase that exists in the cell membrane of neutrophils and con tains p47 phox in the enzyme complex. Superoxide is closely related to vari ous diseases, including infection, myocardial infarction, and aspiration pn eumonia. Therefore we performed this study to establish how lidocaine acts on NADPH oxidase. We examined the effects of lidocaine on superoxide produc tion in neutrophils using the method of 2-methyl-6-phenyl-3,7-dihydro [1,2- a] pyrazin-3-one (CLA phenyl)-dependent chemiluminescence activated by opso nized zymosan (OZ). Treatment with lidocaine 15 muM suppressed OZ-activated CLA phenyl-dependent chemiluminescence by approximately 40% compared with that of OZ only-activated neutrophils. It was further demonstrated that the suppressive effect of lidocaine on superoxide production was dependent on the concentration of lidocaine. Translocation of p47 phox from the cytosol to the cell membrane was studied using immunoblot analysis with a polyclona l anti-p47 phox antibody. Lidocaine suppressed OZ-induced p47 phox transloc ation in a dose-dependent manner. Our results suggest that suppression of s uperoxide production by a therapeutic dose of lidocaine correlates strongly with suppression of p47 phox translocation.