The in vitro reversal of histamine-induced vasodilation in the human internal mammary artery

Citation
A. Tsuda et al., The in vitro reversal of histamine-induced vasodilation in the human internal mammary artery, ANESTH ANAL, 93(6), 2001, pp. 1453-1459
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
Journal title
ANESTHESIA AND ANALGESIA
ISSN journal
0003-2999 → ACNP
Volume
93
Issue
6
Year of publication
2001
Pages
1453 - 1459
Database
ISI
SICI code
0003-2999(200112)93:6<1453:TIVROH>2.0.ZU;2-M
Abstract
Anaphylactic shock therapy includes the use of catecholamines but they may not always be effective. Because vasodilation during anaphylaxis is a resul t of the endothelial release of multiple mediators, we investigated the eff ects of epinephrine, vasopressin, and inhibitors of nitric oxide and prosta noid pathways on histamine-induced relaxation in human internal mammary art ery. The vessel segments were obtained intraoperatively and were suspended in organ chambers to record isometric tension. Norepinephrine (10(-6) M) wa s used to precontract the rings followed by histamine (10(-6.5) M) to relax the vessels and mimic vascular collapse. Epinephrine, vasopressin, methyle ne blue, N-G- monomethyl-L-arginine (L-NMA) and indomethacin were added in a cumulative fashion to reverse the histamine-induced vasodilation. The int ernal mammary artery segments exhibited greater contraction in the presence of the epinephrine (4.9 +/- 0.7g) compared with vasopressin (2.6 +/- 0.7g) . Vasopressin (10(-11) to 10(-7) M), methylene blue (10(-7) to 10(-5) M), L -NMA (10(-6) to 10(-4) M), and indomethacin (10(-7) to 10(-5) M) were only partially effective. These findings suggest that vasopressin and methylene blue may offer a potential therapeutic option in the treatment of histamine -induced vasodilatory shock.