Linear DNA intermediates of the Tto1 retrotransposon in Gag particles accumulated in stressed tobacco and Arabidopsis thaliana

Citation
S. Takeda et al., Linear DNA intermediates of the Tto1 retrotransposon in Gag particles accumulated in stressed tobacco and Arabidopsis thaliana, PLANT J, 28(3), 2001, pp. 307-317
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
28
Issue
3
Year of publication
2001
Pages
307 - 317
Database
ISI
SICI code
0960-7412(200111)28:3<307:LDIOTT>2.0.ZU;2-X
Abstract
The active transcription of some plant retrotransposons under diverse stres s conditions suggests active transposition. However, transposition has been demonstrated only during tissue/cell culture. To examine whether transposi tion is activated under conditions other than tissue/cell culture, DNA inte rmediates for retrotransposition of the tobacco retrotransposon Tto1 were a nalysed. Using transgenic Arabidopsis callus expressing high levels of Tto1 RNA in a ddm1 hypomethylation mutant background, the existence of extrachr omosomal Tto1 linear DNA molecules in a Gag-particle fraction was demonstra ted. By combination with ligation-mediated PCR amplification, we detected T to1 linear DNA molecules in particle fractions from callus and methyl jasmo nate-treated leaves of tobacco, but not from non-stressed leaves. Tto1 DNA intermediates could not be detected in the tobacco corolla where Tto1 is ex pressed. These results indicate that the transcriptional activation of Tto1 by defence-related stresses leads to the synthesis of DNA intermediates, w hereas post-transcriptional suppression of Tto1 activity is suggested in th e corolla.