The measurement of parasite antigen-specific IgE levels using anti-IgE monoclonal antibodies and biotinylated antigens

Citation
M. Takamoto et al., The measurement of parasite antigen-specific IgE levels using anti-IgE monoclonal antibodies and biotinylated antigens, PARASIT RES, 87(11), 2001, pp. 919-923
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
PARASITOLOGY RESEARCH
ISSN journal
0932-0113 → ACNP
Volume
87
Issue
11
Year of publication
2001
Pages
919 - 923
Database
ISI
SICI code
0932-0113(200111)87:11<919:TMOPAI>2.0.ZU;2-5
Abstract
We studied and evaluated an ELISA system, using a sandwich method with a mo noclonal antibody against the Fc domain of IgE molecules and biotinylated a ntigens, to detect parasite antigen-specific IgE quantitatively. The specif ic IgE ELISA titre increases linearly in a dose-dependent manner when the c oncentration of total IgE in samples is less than 2000 ng/ml. Sera from IgE -deficient SJA/9 mice infected with Trichinella spiralis failed to give any measurable IgE. suggesting that other classes of immunoglobulins have no e ffect on this assay. The titre showed a good correlation with PCA titre. A high concentration of the serum from Toxocara canis-infected mice reduced t he T. spiralis-specific IgE ELISA titre. suggesting that the ELISA system i s influenced by a huge amount of IgE against epitopes different from those of target antigens. This ELISA system can also be applied for detecting oth er classes or subclasses of antigen-specific immunoglobulins.