The chromosome partitioning protein, ParB, is required for cytokinesis in Caulobacter crescentus

Citation
Da. Mohl et al., The chromosome partitioning protein, ParB, is required for cytokinesis in Caulobacter crescentus, MOL MICROB, 42(3), 2001, pp. 741-755
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MOLECULAR MICROBIOLOGY
ISSN journal
0950-382X → ACNP
Volume
42
Issue
3
Year of publication
2001
Pages
741 - 755
Database
ISI
SICI code
0950-382X(200111)42:3<741:TCPPPI>2.0.ZU;2-K
Abstract
In Caulobacter crescentus, the genes encoding the chromosome partitioning p roteins, ParA and ParB, are essential. Depletion of ParB resulted in smooth filamentous cells in which DNA replication continued. Immunofluorescence m icroscopy revealed that the formation of FtsZ rings at the mid-cell, the ea rliest molecular event in the initiation of bacterial cell division, was bl ocked in cells lacking ParB. ParB binds sequences near the C. crescentus or igin of replication. Cell cycle experiments show that the formation of bipo larly localized ParB foci, and presumably localization of the origin of rep lication to the cell poles, preceded the formation of FtsZ rings at the mid -cell by 20 min. These results suggest that one major function of ParA and ParB may be to regulate the initiation of cytokinesis in C. crescentus.