Solid-phase synthesis of peptides containing the spin-labeled 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC)

Citation
L. Martin et al., Solid-phase synthesis of peptides containing the spin-labeled 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC), J PEPT RES, 58(5), 2001, pp. 424-432
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF PEPTIDE RESEARCH
ISSN journal
1397-002X → ACNP
Volume
58
Issue
5
Year of publication
2001
Pages
424 - 432
Database
ISI
SICI code
1397-002X(200111)58:5<424:SSOPCT>2.0.ZU;2-W
Abstract
2,2,6,6-Tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) is a nitroxide spin-labeled, achiral C-alpha-tetrasubstituted amino acid recentl y shown to be not only an effective beta -turn and 3(10)/alpha -helix promo ter in peptides, but also an excellent rigid electron paramagnetic resonanc e probe and fluorescence quencher. Here, we demonstrate that TOAC can be ef fectively incorporated into internal positions of peptide sequences using F moc chemistry and solid-phase synthesis in an automated apparatus.