Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics

Citation
Sl. Riley et al., Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics, J MAT S-M M, 12(10-12), 2001, pp. 983-990
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
ISSN journal
0957-4530 → ACNP
Volume
12
Issue
10-12
Year of publication
2001
Pages
983 - 990
Database
ISI
SICI code
0957-4530(2001)12:10-12<983:FOPHAT>2.0.ZU;2-8
Abstract
The limited supply of cartilage tissue with appropriate sizes and shapes ne eded for reconstruction and repair has stimulated research in the area of h ydrogels as scaffolds for cartilage tissue engineering. In this study we de monstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIP N) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimet hacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(e thylene oxide) (PEO) component, and seeded with chondrocytes support cartil age construct growth having nominal thicknesses of 6 mm and relatively unif orm safranin-O stained matrix when cultured statically, unlike constructs g rown with prefabricated macroporous scaffolds. Even though changing the mol ecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the pr ecursor polymer solution, we have demonstrated that it does not appear to a ffect the histological or biochemical characteristics of cartilaginous cons tructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel c ompressive properties increased, as the ratio of the PEGDM:PEO in the hydro gel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen a nd glycosaminoglycan contents per dry weight were highest using the 30:70 P EGDM:PEO formulation (24.4 +/-3.5% and 7.1 +/-0.9%, respectively). The high est equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32 +/-0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue en gineering of cartilage. (C) 2001 Kluwer Academic Publishers.