Separation methods applicable to prostate cancer diagnosis and monitoring therapy

Citation
S. Sumi et al., Separation methods applicable to prostate cancer diagnosis and monitoring therapy, J CHROMAT B, 764(1-2), 2001, pp. 445-455
Citations number
98
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
764
Issue
1-2
Year of publication
2001
Pages
445 - 455
Database
ISI
SICI code
1387-2273(20011125)764:1-2<445:SMATPC>2.0.ZU;2-6
Abstract
During the last decade, significant research has been conducted using prost ate-specific antigen (PSA) in the basic and clinical sciences and many adva nces have occurred in the clinical use of PSA for detecting and monitoring prostate cancer (PCa). Separation methods including gel-permeation chromato graphy, isoelectric focusing lectin-affinity chromatography, polyacrylamide gel electrophoresis and high-performance liquid chromatography have made s ignificant contributions to the discovery and identification of different m olecular forms of PSA. Furthermore, the measurement of free and total PSA h as improved the ability of PSA to detect early PCa. However, unnecessary bi opsies are still needed for men with slightly elevated PSA values. On the o ther hand, PSA is not adequate for staging newly diagnosed PCa and prognost icating the course in individual cases. The possible application of separat ion methods in the basic science of prostate cancer may be associated with identification of more cancer-specific forms of PSA and discoveries of othe r serum proteins useful not only for detecting, but also for staging and pr ognosticating PCa. Such novel markers might lead to a better understanding of PCa aggressiveness and to developments in the clinical field of treatmen t. (C) 2001 Elsevier Science B.V. All rights reserved.