Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-beta 1 production with bone growth of newborn rats

Citation
Zj. Ma et al., Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-beta 1 production with bone growth of newborn rats, INT J MOL M, 8(6), 2001, pp. 623-628
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN journal
1107-3756 → ACNP
Volume
8
Issue
6
Year of publication
2001
Pages
623 - 628
Database
ISI
SICI code
1107-3756(200112)8:6<623:SEOZOI>2.0.ZU;2-O
Abstract
The effect of zinc, an essential trace element, on insulin-like growth fact or-I (IGF-I) and transforming growth factor-beta1 production was investigat ed to determine the role of this metal in bone growth of newborn rats. Femo raldiaphyseal and metaphyseal tissues were obtained between 1 and 28 days a fter birth of newborn rats, and cultured for 24 h in a serum-free Dulbecco' s modified Eagle's medium containing either vehicle or zinc sulfate (10(-6) -10(-4) M). Protein concentration in the medium was significantly increased by culture with bone tissues of newborn rats with increasing age (14 and 2 1 days). Medium IGF-I and TGF-beta1 concentration was gradually reduced wit h increasing age after birth. The presence of zinc (10(-5) and 10(-4) M) ca used a significant increase in protein, IGF-I, and TCTF-beta1 concentration s in the medium cultured with the diaphyseal or metaphyseal tissues obtaine d at 7 and 14 days after birth. The expression of IGF-I and TGF-beta1 mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR ) analysis in the diaphyseal and metaphyseal tissues cultured for 24 h usin g rat IGF-I or TGF-beta1-specific primers. These expressions were significa ntly raised in the presence of zinc (10-4 M) in culture medium. The present study demonstrates that zinc has a stimulatory effect on IGF-I and TGF-bet a1 production in the femoral tissues with bone growth of newborn rats.