Cryopreservation of kiwi shoot tips

Citation
Yj. Wu et al., Cryopreservation of kiwi shoot tips, CRYOLETTER, 22(5), 2001, pp. 277-284
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
CRYOLETTERS
ISSN journal
0143-2044 → ACNP
Volume
22
Issue
5
Year of publication
2001
Pages
277 - 284
Database
ISI
SICI code
0143-2044(200109/10)22:5<277:COKST>2.0.ZU;2-U
Abstract
In this study, the effect of various factors, including the physiological s tate of mother-plants and parameters of the encapsulation-dehydration proto col (cryoprotective treatment, freezing protocol) on the regrowth of shoot tips of kiwi in vitro plantlets was studied. The optimal protocol establish ed was the following: shoot tips were sampled on mother-plants after a one month cold-acclimation period at 5 degreesC. For preculture, which was perf ormed at 5 degreesC, encapsulated shoot tips were transferred at 24-hour in tervals on solid media with increasing sucrose concentrations, from 0.5 to 1.0M. Beads were then desiccated to 26% moisture content (fresh weight basi s), prefrozen from 0 degreesC to -40 degreesC at 0.2 degreesC/min and immer sed rapidly in liquid nitrogen. No differences were noted between in vitro plantlets produced from cryopreserved and control shoot tips as regards lea f color, average height, multiplication rate and peroxidase zymogram patter n. Apices of 3 kiwi accessions were frozen using the above protocol with re growth percentages ranging between 22 and 56%.