PTEN gene mutations in colorectal cancers displaying microsatellite instability

Shin, KH Park, YJ Park, JG

Kh. Shin et al., PTEN gene mutations in colorectal cancers displaying microsatellite instability, CANCER LETT, 174(2), 2001, pp. 189-194

21

INGLESE

Article

Onconogenesis & Cancer Research

CANCER LETTERS

0304-3835 → ACNP

174

2

2001

189 - 194

ISI

0304-3835(200112)174:2<189:PGMICC>2.0.ZU;2-3

To determine the relationship between the mutation of the PTEN gene and gen omic instability in human colorectal. cancer, we screened the PTEN gene in 32 colorectal cancers (eight cell lines and 24 tissues) displaying microsat ellite instability (MSI) and 32 colorectal cancers (six cell lines and 26 t issues) displaying microsatellite stability (MSS). Of 64 samples, six frame shift mutations were identified in six MSI colorectal cancers (two cell lin es and four tumor tissues) but not in MSS colorectal cancers. Of the six MS I colorectal cancers containing PTEN mutation, two case (one cell line and one tumor tissue) showed biallelic mutation of PTEN. Furthermore, to compar e the frequency of PTEN mutation with that of other MSI target genes, the m ononucleotide repeat sequences of TGF-beta RII, BAX, IGFIIR, hMSH6 and hMSH 3 were analyzed. The frequencies of frameshift mutation were revealed 78% i n TGF-beta RII, 56% in BAX, 46.9% in hMSH3, 37.5% in hMSH6,21.9% in IGFIIR, and 18.8% in PTEN. These results suggest that the PTEN gene is a target of genomic instability in MSI colorectal tumorigenesis. (C) 2001 Elsevier Sci ence Ireland Ltd. All rights reserved.