Inhibitory effect of mandarin juice rich in beta-cryptoxanthin and hesperidin on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced pulmonary tumorigenesis in mice

Kohno, H Taima, M Sumida, T Azuma, Y Ogawa, H Tanaka, T

H. Kohno et al., Inhibitory effect of mandarin juice rich in beta-cryptoxanthin and hesperidin on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced pulmonary tumorigenesis in mice, CANCER LETT, 174(2), 2001, pp. 141-150

45

INGLESE

Article

Onconogenesis & Cancer Research

CANCER LETTERS

0304-3835 → ACNP

174

2

2001

141 - 150

ISI

0304-3835(200112)174:2<141:IEOMJR>2.0.ZU;2-7

Previously we reported that a commercial Satsuma mandarin (Citrus unshiu Ma rc.) juice (MJ), MJ2 and MJ5, especially MJ5, effectively suppressed chemic ally-induced rat colon carcinogenesis (Int. J. Cancer 88 (2000) 146). MJ2 a nd MJ5 prepared from MJ have higher amounts of beta -cryptoxanthin and hesp eridin than MJ, suggesting that principle chemopreventive factors in MJs ma y be beta -cryptoxanthin and hesperidin. Present study was conducted to tes t whether these MJs could modify carcinogenesis. in other organ, lung initi ated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in male A/J mice. Mice were,given an intraperitoneal injection of NNK (10 mu mol in sal ine/mouse) to induce pulmonary neoplasms. They also received MJ, MJ2 or MJ5 as a drinking water at night for 21 weeks, starting 1 week after the NNIK injection. Treatments with MJ, MJ2, and MJ5 reduced the incidence of lung t umors and the inhibition by MJ5 (29% reduction) was statistically significa nt (P < 0.05). MJs treatment lowered the multiplicity of lung neoplasms wit hout statistical significance. Immunohistochemically, MJs, especially MJ5, reduced proliferating cell nuclear antigen (PCNA)-positive index in the lun g tumors without affecting PCNA index in hyperplastic alveolar cell lesions . These findings might suggest that MJ5, which contain 3.9 mg beta -cryptox anthin and 100 mg hesperidin in 100 g sample), has chemopreventive ability against NNK-induced mouse lung tumorigenesis. (C) 2001 Elsevier Science Ire land Ltd. All rights reserved.