Loss of heterozygosity of nucleotide excision repair factors in sporadic ovarian, colon and lung carcinomas: implication for their roles of carcinogenesis in human solid tumors

Takebayashi, Y Nakayama, K Kanzaki, A Miyashita, H Ogura, O Mori, S Mutoh, M Miyazaki, K Fukumoto, M Pommier, Y

Y. Takebayashi et al., Loss of heterozygosity of nucleotide excision repair factors in sporadic ovarian, colon and lung carcinomas: implication for their roles of carcinogenesis in human solid tumors, CANCER LETT, 174(2), 2001, pp. 115-125

29

INGLESE

Article

Onconogenesis & Cancer Research

CANCER LETTERS

0304-3835 → ACNP

174

2

2001

115 - 125

ISI

0304-3835(200112)174:2<115:LOHONE>2.0.ZU;2-F

The deficiencies of nucleotide excision repair (NER) factors are genetic di seases, xeroderma pigmentosum (XP) increasing risk of developing cancer on sun-exposed areas of the skin. However, the abnormality of NTR factors in h uman sporadic carcinoma remains unclear. Loss of heterozygosity (LOH) analy sis for the XP, XPA, XPB, XPC, XPD, XPE, XPF, XPG and the transcription-cou pled repair factor, Cockayne syndrome B (CSB) revealed that NER factors wer e abnormal in 62.1% of ovarian tumors (18/29), 16.7% of colon (2/12) and 22 .2% lung (2/9) carcinomas. Furthermore, 13.8% of ovarian, 8.3% of colon and 22% of lung carcinomas exhibited LOH for NER factors without LOH for tumor suppressor genes such as p53, FH1T, APC, BRCA1, BRCA2 and DCC. Although bo th microsatellite instability and LOH of NER factors were observed in some cases, there was no strong association between them in the present study. T hese observations raise the possibility that alterations of NER factors may be frequent in human sporadic carcinomas. Further study should be needed t o find the direct evidence of NER gene abnormalities in human sporadic carc inoma tissues. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.