Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor

Citation
Iv. Broek et al., Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor, BR J CANC, 85(9), 2001, pp. 1387-1395
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
BRITISH JOURNAL OF CANCER
ISSN journal
0007-0920 → ACNP
Volume
85
Issue
9
Year of publication
2001
Pages
1387 - 1395
Database
ISI
SICI code
0007-0920(20011102)85:9<1387:LMOMMC>2.0.ZU;2-P
Abstract
The 67 kD laminin receptor (67LR) binds laminin-1 (LN), major component of the basement membrane, with high affinity. In this study, we demonstrated t hat human multiple myeloma cell lines (HMCL) and murine 5T2MM cells express 67LR. CD38(bright+), plasma cells in fresh multiple myeloma (MM) bone marr ow (BM) samples showed weaker 67LR expression, but expression increased aft er direct exposure to a BM endothelial cell line (4LHBMEC). LN stimulated t he in vitro migration of 3 HMCL (MM5.1, U266 and MMS.1), primary MM cells a nd the murine 5T2MM cells. 67LR has been shown to mediate the actions of LN through binding to CDPGYIGSR, a 9 amino acid sequence from the B1 chain of LN. MM cell migration was partially blocked by peptide 11, a synthetic non apeptide derived from this amino sequence and also by a blocking antiserum against 67LR. Co-injection of peptide 11 with 5T2MM cells in a murine in vi vo model of MM resulted in a decreased homing of 5T2MM cells to the BM comp artment. In conclusion, LN acts as a chemoattractant for MM cells by intera ction with 67LR. This interaction might be important during extravasation o f circulating MM cells. (C) 2001 Cancer Research Campaign.