The US2 gene product of herpes simplex virus type 2 interacts with cytokeratin 18

Citation
F. Goshima et al., The US2 gene product of herpes simplex virus type 2 interacts with cytokeratin 18, ARCH VIROL, 146(11), 2001, pp. 2201-2209
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
0304-8608 → ACNP
Volume
146
Issue
11
Year of publication
2001
Pages
2201 - 2209
Database
ISI
SICI code
0304-8608(2001)146:11<2201:TUGPOH>2.0.ZU;2-H
Abstract
In order to clarify the biological role of US2 gene product of herpes simpl ex virus type 2 (HSV-2), a HeLa cDNA library was screened,in the yeast two- hybrid system using US2 protein as bait, and several interacting proteins w ere identified, including cytokeratin 18. US2 protein was co-immunoprecipit ated with cytokeratin 18 from HSV-2 infected cell lysates. Analysis of infe cted or A431 cells by immunofluorescence showed that US2 protein gave filam entous or dot-like cytoplasmic staining pattern, and that it co-localized w ith cytokeratin 18. When US2 protein was expressed alone, it co-localized w ith cytokeratin 18. To define the domain interacting with cytokeratin 18, d eletion mutant proteins were constructed and cells transfected with mutants were analyzed by indirect immunofluorescence. These results suggest that t he N-terminal half of the US2 protein, especially the region containing ami no acids 42-77, is important for interaction with cytokeratin 18.