Functional analysis of the Streptomyces lividans type I signal peptidases

Citation
N. Geukens et al., Functional analysis of the Streptomyces lividans type I signal peptidases, ARCH MICROB, 176(5), 2001, pp. 377-380
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
176
Issue
5
Year of publication
2001
Pages
377 - 380
Database
ISI
SICI code
0302-8933(200111)176:5<377:FAOTSL>2.0.ZU;2-Q
Abstract
Type I signal peptidases are responsible for the proteolytic cleavage of th e signal peptide of secreted proteins. In the gram-positive bacterium Strep tomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isol ated encoding putative type I signal peptidases. In this work, the differen t sip genes were cloned and expressed. Subsequently, the Sip proteins were purified to raise antibodies. Although the four Sip proteins share a low de gree of sequence similarity and differ significantly in size and pI, anti-S ip antibodies cross-reacted intensively. Functional signal peptidase proces sing activity for each of these Sip proteins was shown both in vitro and in vivo. The different Sip proteins did not exhibit the same cleavage efficie ncy on the Bacillus subtilis pre-chitosanase.