Rubrobacter-related bacteria associated with rosy discolouration of masonry and lime wall paintings

Citation
C. Schabereiter-gurtner et al., Rubrobacter-related bacteria associated with rosy discolouration of masonry and lime wall paintings, ARCH MICROB, 176(5), 2001, pp. 347-354
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
176
Issue
5
Year of publication
2001
Pages
347 - 354
Database
ISI
SICI code
0302-8933(200111)176:5<347:RBAWRD>2.0.ZU;2-D
Abstract
A molecular approach was chosen to analyse the correlation between bacteria l colonisation and rosy discolouration of masonry and lime wall paintings o f two historically important buildings in Austria and Germany. The applied molecular method included PCR amplification of genes encoding the small sub unit rRNA of bacteria (16S rDNA), genetic fingerprinting by denaturing grad ient gel electrophoresis (DGGE), construction of 16S rDNA clone libraries, and comparative phylogenetic sequence analyses. The bacterial community of one red-pigmented biofilm sampled in Herberstein (Austria) contained bacter ia phylogenetically related to the genera Saccharopolyspora, Nocardioides, Pseudonocardia, Rubrobacter, and to a Kineococcus-like bacterium. The bacte rial community of the second red-pigmented biofilm sampled in Herberstein c ontained bacteria related to Arthrobacter, Comamonas, and to Rubrobacter. R ubrobacter-related 16S rDNA sequences were the most abundant. In the red-pi gmented biofilm sampled in Burggen (Germany), only Rubrobacter-related bact eria were identified. No Rubrobacter-related bacteria were detected in non- rosy biofilms. The majority of sequences (70%) obtained from the bacterial communities of the three investigated rosy biofilms were related to sequenc es of the genus Rubrobacter (red-pigmented bacteria), demonstrating a corre lation between Rubrobacter-related bacteria and the phenomenon of rosy disc olouration of masonry and lime wall paintings.