Enzyme activity changes during anthocyanin synthesis in 'Olympia' grape berries

Citation
S. Hiratsuka et al., Enzyme activity changes during anthocyanin synthesis in 'Olympia' grape berries, SCI HORT A, 90(3-4), 2001, pp. 255-264
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
SCIENTIA HORTICULTURAE
ISSN journal
0304-4238 → ACNP
Volume
90
Issue
3-4
Year of publication
2001
Pages
255 - 264
Database
ISI
SICI code
0304-4238(20011116)90:3-4<255:EACDAS>2.0.ZU;2-2
Abstract
Anthocyanin synthesis began in the skin of 'Olympia' (Vitis labruscana Bail ey) grape when the berry growth reached a late stage in the second sigmoid growth-curve. During berry development, shikimate NADP oxidoreductase (SORa se, EC.1.1.1.25), phenylalanine ammonia-lyase (PAL, EC.4.3.1.5), and chalco ne flavanone isomerase (CM, EC.5.5.1.6) were investigated in the skin. Thes e enzyme activities were high during early development of the young berry, but rapidly decreased thereafter, and were activated again when rapid antho cyanin synthesis subsequently occurred. When PAL from the young berry was a nalyzed by isoelectricfocusing polyacrylamide gel electrophoresis (IEF-PAGE ), it showed a single activity band with a pI value of approximately 5.4, w hereas PAL from the ripe berry showed two activity bands with pIs of 5.4 an d 6.5. Thus, SORase, PAL, and CHFI seem to be involved in anthocyanin accum ulation simultaneously, and newly synthesized isozyme of PAL may play a key role in pigment formation in 'Olympia' skin. The function of these enzyme activities in young berry is also discussed in this text. (C) 2001 Elsevier Science B.V. All rights reserved.